NR1H4 Knockout cell line(Hep 3B)

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location： Home > Products > KO Cell Lines > NR1H4 Knockout cell line(Hep 3B)

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NR1H4 Knockout cell line(Hep 3B) Order

NR1H4 Knockout cell line(Hep 3B)
Catalog#
YKO-H066
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NR1H4 Knockout cell line(Hep 3B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NR1H4 Knockout cell line (Hep 3B)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
NR1H4
Gene ID
9971
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
87%MEM+10% FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Target region
Knockout region: Exon 4 (based on transcript NR1H4-203)
Validation: PCR+Sanger Sequencing
NR1H4 Knockout cell line(Hep 3B) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hep 3B		 STR Info (Cell bank)
Cell Line:HEP-3B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 12141214
D7S820 810810
D16S539 10101010
VWA 17171717
TH01 6767
AMEL XXXX
TPOX 9999
CSF1PO 8888
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NR1H4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep 3B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NR1H4 gene knockout NR1H4 Knockout cell line (Hep 3B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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